Applying Real-Time Quantitative PCR to Fusarium Crown Rot of Wheat
نویسندگان
چکیده
منابع مشابه
First report of Fusarium chlamydosporum causing crown rot and dumping of on Durum wheat in Algeria
Several species of Fusarium infect durum wheat plants in the eastern part of Algeria. Endophytic fungus from random wheat seeds were sampled from fifty locations in eastern Algeria and shows the existence of Fusarium chlamydosporum regarding the macroscopic and microscopic characteristics. Molecular identification using EF1 and ITS1 primers were confirmed the presence of Fusarium chlamydosporum...
متن کاملPhases of infection and gene expression of Fusarium graminearum during crown rot disease of wheat.
Fusarium graminearum causes head blight (FHB) and crown rot (CR) diseases in wheat. Compared with FHB, CR symptom development occurs slowly, usually taking 4 to 8 weeks to become visible. To characterize CR development, we used histological and real-time quantitative polymerase chain reaction analyses to assess fungal colonization during a timecourse of infection. Three distinct phases of infec...
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Crown rot is one of the main important fungal diseases affecting wheat in many areas of the world, including Australia, USA, and Iran. Until now, there had been no report of this pathogen in Iraq. Plants displaying crown rot symptoms were observed in Shaat Alarab (Basra, Iraq); we investigated the causal agent of the disease. Samples were surface-sterilized in bleach (1% available chlorine...
متن کاملQuantitative Real-Time PCR
Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...
متن کاملReal time quantitative PCR.
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over conta...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2007
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-91-8-1021